Abstract

Deterioration of water quality is commonly found in secondary water supply systems (SWSSs), especially the growth of microbes. To explore the metabolic mechanism for rapid microbial regrowth in SWSSs, a regrowth potential assessment, flow cytometry, and quantitative PCR were conducted. Metagenomic and 16S rRNA gene sequencing were performed to better understand the microbial communities and metabolism. It was found that the increased biomass in the SWSS was significantly higher than that in the drinking water distribution system (DWDS). Statistical analysis revealed that ammonia oxidation was the dominant driver of increased biomass in the SWSS. The abundances of ammonia oxidation bacteria, concentration of nitrogen species, and related enzymes demonstrated that ammonia oxidation in the SWSS was more vigorous than that in the DWDS. In the SWSS, the metabolism of the ammonia oxidation cluster was more vigorous, and ammonia-oxidizing bacteria (AOB) were the dominant nitrifying bacteria . Incomplete nitrification products were involved in the metabolism of heterotrophic bacteria and promoted the growth of heterotrophic bacteria in the SWSS. More attention should be given to controlling incomplete nitrification to improve tap water quality.