茶树LBD转录因子CsLBD39的克隆鉴定及在硝酸盐含量调控中的功能研究

Abstract

Nitrate nitrogen is an important nitrogen source for tea plants’ growth and development. LBD transcription factors play important roles in response to the presence of nitrate in plants. The functional study of LBD transcription factors in tea plants remains limited. In this study, the LBD family geneCsLBD39was isolated and characterized from tea plants. Sequence analysis indicated that CsLBD39 contained a highly conserved CX2CX6CX3CX domain. The phylogenetic tree assay showed that CsLBD39 belonged to class II subfamily of the LBD family.CsLBD39was highly expressed in flowers and root; we determined that its expression could be induced by nitrate treatment. The CsLBD39 protein was located in the nucleus and has transcriptional activation activity in yeast. Compared with the wild type, overexpression ofCsLBD39gene inArabidopsisresulted in smaller rosettes, shorter main roots, reduced lateral roots and lower plant weights. The nitrate content and the expression levels of genes related to nitrate transport and regulation were decreased in transgenicArabidopsishostingCsLBD39gene. Compared with the wild type,CsLBD39overexpression in transgenicArabidopsishad smaller cell structure of leaves, shorter diameter of stem cross section, and slender and compact cell of stem longitudinal section. Under KNO3treatment, the contents of nitrate, anthocyanins, and chlorophyll in leaves, and the content of nitrate in roots ofArabidopsisoverexpressingCsLBD39were reduced, the expression levels of nitrate transport and regulation related genes were decreased. The results revealed that CsLBD39 may be involved in nitrate signal transduction in tea plants as a negative regulator and laid the groundwork for future studies into the mechanism of nitrate response.Keywords:tea plant;nitrate;expression level;transcription factor;overexpression