Protocol to identify small molecules promoting rat and mouse cardiomyocyte proliferation based on the FUCCI and MADM reporters
Lixia Zheng, Zihao Wang, Jianyong Du, Xiaojun Zhu, Jing-Wei Xiong
Journal：STAR Protocols
IF：0
DOI：10.1016/j.xpro.2022.101903
PMID：
Published：2022-12-05
research field：药理学细胞生物学心脏病学
Abstract
Summary Discovery of small molecules promoting cardiomyocyte proliferation is important for heart regeneration and related heart disease. Here, we describe a protocol to isolate neonatal rat and mouse cardiomyocytes, infect cardiomyocytes with Tnnt2-mAG-hGeminin (1/110) or Tnnt2-Cre adenovirus, and identify small molecules that promote cardiomyocyte proliferation by high-content microscopy. This protocol can be modified to investigate other pro-proliferation factors in cardiomyocytes and other cell types. For complete details on the use and execution of this protocol, please refer to Du et al. (2022).1
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