Generation of a homozygous ABCA7-knockout human iPSC line using the CRISPR/Cas9 system
Mingfeng Guan, Yue Chai, Ran Yi, Yuewen Chen, Jacque P.K. Ip, Tao Ye, Yu Chen
Journal：Stem Cell Research
IF：1.59
DOI：10.1016/j.scr.2022.103000
PMID：36521383
Published：2022-12-07
research field：神经科学分子生物学干细胞研究遗传学
Abstract
ABCA7 encodes an ATP-binding cassette transporter, and its loss-of-function variants are associated with Alzheimer’s disease. To investigate the role of ABCA7 deficiency in the pathogenesis of Alzheimer’s disease, we generated a homozygous ABCA7 -knockout induced pluripotent stem cell (iPSC) line using CRISPR/Cas9-mediated gene editing. This ABCA7 -deficient iPSC line maintains a normal karyotype, expression of pluripotency markers, and trilineage differentiation capacity.
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