This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of IKK alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with IKK alpha antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using IKK alpha antibody , Top panel: wild-type (WT); Bottom panal: IKK alpha shRNA knockdown (KD). Nuclei were stained blue with DAPI; IKK alpha was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of IKK alpha expression in HepG2 cells using IKK alpha antibody . Green, isotype control; red, IKK alpha.

Immunocytochemical staining of HepG2 cells with component of inhibitor of IKK alpha antibody . Nuclei were stained blue with DAPI; Component of inhibitor of IKK alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: medium. Scale bar, 20 μm.

Western blotting analysis using IKK alpha antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IKK alpha antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using IKK alpha antibody . IKK alpha expression in wild type (WT) and IKK alpha shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with IKK alpha antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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