This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of forkhead box A1 expression in HepG2 cells using forkhead box A1 antibody . Green, isotype control; red, forkhead box A1.

Immunocytochemical staining of HepG2 cells with Forkhead box A1 antibody . Nuclei were stained blue with DAPI; Forkhead box A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Forkhead box A1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Forkhead box A1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using forkhead box A1 antibody . Forkhead box A1 expression in wild-type (WT) and forkhead box A1 (FOXA1) shRNA knockdown (KD) HepG2 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with forkhead box A1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of forkhead box A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with forkhead box A1 antibody and analyzed using BD flow cytometer.

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