The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of CLPP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CLPP antibody and analyzed using CytoFLEX.

Immunocytochemical staining of HeLa cells using CLPP antibody , Top panel: wild-type (WT); Bottom panal: CLPP shRNA knockdown (KD). Nuclei were stained blue with DAPI; CLPP was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of CLPP expression in HepG2 cells using CLPP antibody . Green, isotype control; red, CLPP.

Immunocytochemical staining of HepG2 cells with CLPP antibody . Nuclei were stained blue with DAPI; CLPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using CLPP antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLPP antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . CLPP, caseinolytic mitochondrial matrix peptidase proteolytic subunit.

Western blotting analysis using CLPP antibody . CLPP expression in wild type (WT) and CLPP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CLPP antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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