Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Western blotting analysis using Integrin beta-1/CD29 antibody . Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 knockout (KO) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Integrin beta-1/CD29 expression in HT-1080 cells using Integrin beta-1/CD29 antibody . Green, isotype control; red, Integrin beta-1/CD29.

Immunocytochemical staining of HT-1080 cells with Integrin beta-1/CD29 antibody . Nuclei were stained blue with DAPI; Integrin beta-1/CD29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human bladder carcinoma using integrin beta-1/CD29 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using Integrin β1/CD29 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β1/CD29 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Integrin beta-1/CD29 antibody . Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 knockout (KO) HeLa cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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