This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.

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Validation of EHMT2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with EHMT2 antibody and analyzed using CytoFLEX.

Immunocytochemical staining of HeLa cells using EHMT2 antibody , Top panel: wild-type (WT); Bottom panal: EHMT2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of EHMT2 expression in HepG2 cells using EHMT2 antibody . Green, isotype control; red, EHMT2 .

Immunocytochemical staining of HepG2 cells with EHMT2 antibody . Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Western blotting analysis using EHMT2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EHMT2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using EHMT2 antibody . EHMT2 expression in wild type (WT) and EHMT2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with EHMT2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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