This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

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Immunocytochemical staining of HT-1080 cells using PRKACA antibody , Top panel: wild-type (WT); Bottom panal: PRKACA shRNA knockdown (KD). Nuclei were stained blue with DAPI; PRKACA was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of cAMP Protein Kinase Catalytic Subunit expression in C2C12 cells using cAMP Protein Kinase Catalytic Subunit antibody . Green, isotype control; red, cAMP Protein Kinase Catalytic Subunit.

Immunocytochemical staining of HeLa cells with cAMP Protein Kinase Catalytic Subunit antibody . Nuclei were stained blue with DAPI; cAMP Protein Kinase Catalytic Subunit was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using cAMP Protein Kinase Catalytic Subunit antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cAMP Protein Kinase Catalytic Subunit antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using cAMP Protein Kinase Catalytic Subunit antibody . cAMP Protein Kinase Catalytic Subunit expression in wild type (WT) and cAMP Protein Kinase Catalytic Subunit shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with cAMP Protein Kinase Catalytic Subunit antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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