南美超级胎牛血清999尝鲜特惠
永生化心外膜细胞系的建立与特征鉴定
Establishment and characterization of an immortalized epicardial cell line
Haobin Jiang, Shen Song, Jiacheng Li, Qianqian Yin, Shengshou Hu, Yu Nie
Journal：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
IF：5.31
DOI：10.1111/jcmm.16496
PMID：33822475
Published：2021-04-06
research field：分子生物学进化生物学微生物学
Abstract
Recently, the increasing significance of the epicardium in cardiac development and regeneration is beginning to be recognized. However, because of the small proportion of primary epicardial cells and the limited cell culture time, further research on the mechanism of epicardial cells is hindered. Here, we transfected simian virus 40 Large T (SV40-LT) into primary epicardial cells to establish an immortalized cell line, named EpiSV40. We further demonstrated that EpiSV40 can be easy to culture and has the proliferation, migration and differentiation capacities comparable to primary epicardial cells. EpiSV40 can serve as an ideal in vitro model for epicardial cell research, which will booster the study of the epicardium in cardiac development and heart regeneration.
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