本产品是2×Mix预混合试剂，能够实现在单个反应孔中进行多重荧光定量PCR。本产品含有基因改造的热启动Taq酶，极大地提高了扩增灵敏度和特异性，本产品对多重反应缓冲体系进行了深度优化，能够提高反应的扩增效率，促进低浓度模板的有效扩增。本产品可用于基因分型和基因多重定量分析。
产品组分
| 组分编号 | 组分名称 | 11208ES03 | 11208ES06 | 11208ES08 | 11208ES60 |
|---|---|---|---|---|---|
| 11208-A | 2×Hieff Unicon® TaqMan Multiplex qPCR Master Mix | 1 mL | 5 × 1 mL | 5 mL | 20 × 5 mL |
| 11208-B | 50×Low Rox | 40 μL | 200 μL | 200 μL | 20 × 200 μL |
| 11208-C | 50×High Rox | 40 μL | 200 μL | 200 μL | 20 × 200 μL |
- 灵敏度高
冰袋运输。-20°C储存，有效期18个月。
Q：11208 跟 11205 有什么区别？
A：酶不一样，11208 是可以做多重PCR，11205 只能做单重PCR，做qPCR 是没有问题的。
Q：11208 的qPCR 反应体系可以用 5 μL/10 μL 吗？
A：可以，但一般推荐 20ul。大体系减少误差。5μl 体系太小，加样误差会很大，不推荐。
Q：11208 可扩增多长的产物啊？
A：建议在 300bp 以下，过长的产物会导致酶效降低。若一定要用，建议用三步法。
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