性能参数
表达区间及表达系统(Source) |
Human PAI-1 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val24-Pro402.[Accession | P05121-1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 43.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE and HPLC |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) | 冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。将冻干的蛋白质溶解在50mM NaAC、0.1M NaCl、100mM L-精氨酸(pH 5.5)中。复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
1.为了您的安全和健康,请穿实验服并戴一次性手套操作。
2.本产品仅作科研用途。
Ver. CN20230625