High-mobility Group Box 1 protein (HMGB1), also known as HMG1 or Amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity. HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide. HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis. HMGB1 is widely recognized as a multifunctional alarmin that stimulates inflammation upon sterile or infectious insult. It is implicated in the pathogenesis of a broad range of diseases including atherosclerosis, sepsis, cancer, neurodegenerative diseases, and autoimmunity.

高纯度、高活性、低内毒素、高批间一致性

SDS-PAGE

Figure 1. Human HMGB1 on SDS-PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

Figure 2. The purity of Human HMGB1 is greater than 95% as determined by SEC-HPLC.

 SPR Data

Human AGER, His Tag immobilized on CM5 Chip can bind Human HMGB1, His Tag with an affinity constant of 0.19 μM as determined in SPR assay.

-25 ~ -15℃保存，收到货之后有效期1年。
复溶后， 无菌条件下，-85 ~ -65℃保存，3个月有效期。