性能参数
蛋白别名(Synonyms) |
CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR |
种属(Species) |
Human |
表达系统(Source) |
HEK293 |
表达序列(Sequence) |
Gln31-Thr334 |
Accession |
Q15223-1 |
标签(Tag) |
C-hFc |
分子量(Molecular Weight) |
The protein has a predicted MW of 59.89 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Bis-Tris PAGE; |
活性(Activity) |
ELISA Data: Immobilized Human Nectin-3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA .\r\n \r\n ELISA Data: Immobilized Human Nectin-4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 0.27μg/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
Ver. CN20240905