RalBP1 interacts with RalA and the endocytosis protein REPS2 (POB1) through its carboxy-terminal Ral binding domain. RalBP1 has an intrinsic GTPase activating function and interacts with Cdc42 through its centrally located Rho-GAP domain. A protein complex containing RalBP1/POB1/RalA regulates endocytosis of membrane receptors. RalBP1 also functions as a non-ABC transporter that catalyzes the ATP-dependent transport of numerous xenobiotics, including glutathione conjugates and some chemotherapeutic agents.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100

Western blot

Western blot analysis of RALBP1 in HeLa(WT) and RALBP1-Knockout HeLa(KO) using RALBP1 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Adenocarcinoma of ovary tissue using RALBP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-15到-25度