During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200  IP: 1/20  FC: 1/50-1/100

Western

Western blot analysis of Phospho-POLR2A (S5) in (1) HeLa lysates treated with Lambda Phosphatase; (2) HeLa lysates using Phospho-RNA Polymerase II Subunit B1 (Ser5) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-POLR2A (S5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Phospho-RNA Polymerase II Subunit B1 (Ser5) in Hela using Phospho-POLR2A (S5) antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。