The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200  IP: 1/20  FC: 1/50-1/100

Western

Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody.

Immunoprecipitation

Immunoprecipitation analysis of MCM2 (2B5) in Hela lysates using MCM2 antibody.

Immunofluorescence

Immunofluorescence analysis of MCM2 (2B5) in Hela cells using MCM2 antibody(green),and DAPI (blue).

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded ovarian cancer tissues using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。