Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2.

推荐稀释比 WB: 1/1000-1/2000  IHC: 1/100-1/200  IF: 1/50-1/200

Western

Western blot analysis of Dnmt1 in HEK293 ，NIH/3T3， C6 lysates using Dnmt1 antibody.

Immunohistochemistry of paraffin-embedded human placental tissue section using the antibody Dnmt1 Rabbit mAb at a dilution of 1/50.Heat-induced epitope retrieval was performed using Tris-EDTA buffer (pH 9.0).

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。