The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Immunocytochemistry

Immunocytochemistry analysis of Bid (green) in Hela using Bid antibody,and DAPI(blue).

Western

Western blot analysis of Bid in Hela, A549, HL-60 lysates using Bid antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded liver cancer tissue using Bid antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.