Atg9, one of the Atg proteins identified in yeast, is essential for autophagosome formation. There are two human functional orthologues based on the yeast homolog Atg9p: Atg9A, which has also been identified as Atg9L1 and mAtg9, and Atg9L2, which was first reported as nitric-oxide synthase 3 antisense (NOS3AS). Atg9A is an integral membrane protein that is required for both the initiation and the expansion of the autophagosome.

Western

Western blot analysis of ATG9A in HepG2 lysates using ATG9A antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ATG9A antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of ATG9A in Hepg2 using ATG9A antibody.