性能参数
蛋白别名(Synonyms) |
Sema III; Semaphorin; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL; |
种属(Species) |
Human |
表达系统(Source) |
HEK293 |
表达序列(Sequence) |
Asn21-Val771 |
Accession |
Q14563 |
标签(Tag) |
N-His |
分子量(Molecular Weight) |
The protein has a predicted MW of 87.7 kDa. Due to cleavage site, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Bis-Tris PAGE |
活性(Activity) |
ELISA Data: Immobilized Anti-SEMA3A Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30μg/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外观(Appearance) |
冻干粉 |
储存条件
-25~-15℃保存,收到货之后有效期1年。复溶后, 2 ~8 °C保存,7天有效期。复溶后, -85~-65℃保存,3个月有效期。
复溶方法
开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
注意事项
Ver. CN20240905