产品说明书
FAQ
COA
已发表文献
ILT4, also known as CD85d, LIR-2, and LILRB2, is closely related to mouse PIR-B and is primarily expressed on monocytes, dendritic cells, and neutrophils. The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64% aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules. It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1. Ligation of ILT4 induces tyrosine phosphorylation within its cytoplasmic ITIMs, association with SHP-1, and inhibition of stimulatory signaling. ILT4 activation promotes the development of tolerogenic dendritic cells and the subsequent induction of regulatory T cells and CD4+ T cell anergy.
产品性质
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别名（Synonyms） |
CD85d, ILT4, ILT-4, ILT4CD85d, LILRB2, LIR2, MIR10 |
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Uniprot No. |
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表达区间及表达系统（Source） |
Recombinant Human LILRB2 / CD85d / ILT4 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Gln22-His458. |
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分子量(Molecular Weight) |
Approximately 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
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外观(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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纯度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
1. SPR Data: Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20 nM as determined in SPR assay (Biacore T200). 2. SPR Data: Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag with an affinity constant of 0.14 μM as determined in a SPR assay (Biacore T200). |
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内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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复溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
Tris-Bis PAGE
HB220707
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