Flotetuzumab (MGD006; S80880) 是一种双特异性 CD123/CD3 双亲和再靶向 (DART) 抗体。Flotetuzumab 通过同时结合靶细胞的 CD123 和效应 T 细胞的 CD3，而重新激活 T 细胞，导致 T 细胞介导的靶细胞的细胞毒性。Flotetuzumab 对小鼠中急性髓系白血病 (AML) 患者来源异种移植物 (PDX) 模型具有抑制效力。

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The detected molecular weight of Anti-CD123 & CD3 Reference Antibody (Flotetuzumab) is 58.91 kDa.

Anti-CD123 & CD3 Reference Antibody (Flotetuzumab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-CD123 & CD3 Reference Antibody (Flotetuzumab) is 91.49%, determined by SEC-HPLC.

Flotetuzumab bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies (PE Streptavidin ) , and test by flow cytometry. As shown in fig, Flotetuzumab bound to huCD3e-jurkat cells, and the EC50 was 33.800 nM.

Flotetuzumab-Biotin bound to GSV0-hu-CD123-ECD-Fc cells, and then rebounded to fluorescent secondary antibodies (PE Streptavidin ) , and test by flow cytometry. As shown in fig, FlotetFlotetuzumab-Biotin bound to GSV0-hu-CD123-ECD-Fc cells, and the EC50 was 31.24 nM.

Co-incubation of Flotetuzumab with Jurkat cells, then with the addition of GSV0-hu-CD123-ECD-Fc cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.. As shown in fig, Flotetuzumab was able to activate the NF-AT signaling pathway, and the EC50 was 4.555 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.