M701 是一种人源化双特异性 CD3/EpCAM T 细胞衔接抗体。M701 可有效杀灭高表达 EpCAM 的癌细胞。M701 可用于恶性腹水和胃癌的研究。

For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

The detected molecular weight of Anti-CD3 & EpCAM Reference Antibody (M701) is 125.71 kDa.

Anti-CD3 & EpCAM Reference Antibody (M701) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-CD3 & EpCAM Reference Antibody (M701) is 95.5%, determined by SEC-HPLC.

M701A bound to EpCAM protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, M701A bound to in hu-EpCAM-His, and the EC50 was 0.013 nM.

M701A bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, M701A bound to huCD3e-jurkat cells, and the EC50 was 3.809 nM.

Co-incubation of M701A with Jurkat cells, then with the addition of GSV0-hu-EPCAM-ECD-Fc cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, M701A was able to activate the NF-AT signaling pathway, and the EC50 was 0.707 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.