Tebotelimab (MGD-013) 是一种人源化的 IgG4κ 双特异性 PD-1/LAG-3 双亲和再靶向 (DART) 抗体。Tebotelimab 结合 NS0 细胞表面表达的 PD-1 和 LAG-3，EC50 分别为 1.65 nM 和 0.41 nM。Tebotelimab 阻断 PD-1/PD-L1、PD-1/PD-L2 和 LAG-3/HLA (MHC-II) 相互作用和 PD-1 信号。Tebotelimab 可恢复耗尽的 T 细胞功能并增强抗肿瘤免疫力。

For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

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The detected molecular weight of Anti-LAG-3 & PD-1 Reference Antibody (Tebotelimab) is 164.05 kDa.

Anti-LAG-3 & PD-1 Reference Antibody (Tebotelimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-LAG-3 & PD-1 Reference Antibody (Tebotelimab) is 90.76%, determined by SEC-HPLC.

Tebotelimab bound to huLAG-3-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huLAG-3-CHO-K cells, and the EC50 was 8.727 nM.

Tebotelimab bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huPD-1-Jurkatt cells, and the EC50 was 0.47 nM.

Tebotelimab bound to huLAG-3-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huLAG-3-CHO-K cells, and the EC50 was 8.727 nM.

Tebotelimab bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huPD-1-Jurkatt cells, and the EC50 was 0.47 nM.

Co-incubation of Tebotelimab with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Tebotelimab was able to block the PD-1/PD-L1 signaling pathway, and the EC50 was 1.266 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.