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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

Anti-4-1BB & TPBG/5t4 Reference Antibody (Apv-527) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-4-1BB & TPBG/5t4 Reference Antibody (Apv-527) is 98.99%, determined by SEC-HPLC.

Yh32367 bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-4-1BB-His, and the EC50 was 0.007 nM.

Yh32367 bound to Her2 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-Her2-His, and the EC50 was 0.009 nM.

Yh32367 bound to Her2 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-Her2-His, and the EC50 was 0.009 nM.

Yh32367 bound to BT474 cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to BT474 cells, and the EC50 was 1.889 nM.

Yh32367 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to hu4-1BB-CHO-K cells, and the EC50 was 5.701 nM.

Co-incubation of Yh32367 with 4-1BB-NF-κB-Jurkat cells, then with the addition of BT474 cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Yh32367 was able to activate the NF-κB signaling pathway, and the EC50 was 0.355 nM.

Co-incubation of Yh32367 with BT474 cell and PBMCs for 4 hours, then LDH was detected to evaluate the ADCC activity of Yh32367. As shown in fig, Yh32367 has ADCC activity, and the IC50 was 0.054 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.