Volrustomig 是一种抗 CTLA4/PDCD1 的人源 IgG1 κ 单克隆抗体。Volrustomig 的同型对照产品：Human IgG1 kappa, Isotype Control。

For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

The detected molecular weight of Anti-CTLA4 & PD-1 Reference Antibody (Volrustomig) is 146.51 kDa.

Anti-CTLA4 & PD-1 Reference Antibody (Volrustomig) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-CTLA4 & PD-1 Reference Antibody (Volrustomig) is 98.99%, determined by SEC-HPLC.

Volrustomig bound to CTLA4 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Volrustomig bound to huCTLA4-His, and the EC50 was 0.008 nM.

Volrustomig bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Volrustomig bound to hu-PD-1-His, and the EC50 was 0.006 nM.

Volrustomig bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Volrustomig bound to huPD-1-Jurkat cells, and the EC50 was 2.279 nM.

Volrustomig bound to huCD40-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Volrustomig bound to huCD40-CHO-K cells, and the EC50 was 8.874 nM.

Co-incubation of Volrustomig with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Volrustomig was able to block the PD-1/PD-L1 signaling pathway, and the EC50 was 6.643 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.