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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

The detected molecular weight of Anti-CD20 & CD47 Reference Antibody (Amulirafusp alfa) is 173.84 kDa.

Anti-CD20 & CD47 Reference Antibody (Imm0306) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-CD20 & CD47 Reference Antibody (Imm0306) is 99.17%, determined by SEC-HPLC.

Imm0306 bound to CD20 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Imm0306 bound human CD20 VLP, and the EC50 was 0.056 nM.

Imm0306 bound to CD47 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Imm0306 bound human CD47 Protein-His, and the EC50 was 0.015 nM.

Imm0306 bound to CCRF-CEM cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Imm0306 bound to CCRF-CEM.

Imm0306 bound to Raji cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Imm0306 bound to Raji cells, and the EC50 was 1.438 nM.

Co-incubation of Imm0306 with Raji cell and PBMCs for 4 hours, then LDH was detected to evaluate the ADCC activity of Imm0306. As shown in fig, Imm0306 has ADCC activity, and the IC50 was 0.001 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.