Zubotamig 是一种靶向 CD3E/VTCN1 的 Ig(G1 -κ_G1 -λ2) 型嵌合的人源抗体。

For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

The detected molecular weight of Anti-CD3 & B7-H4 Reference Antibody (Gen1047) is 144.74 kDa

Anti-B7-H4 & CD3 Reference Antibody (Gen1047) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-B7-H4 & CD3 Reference Antibody (Gen1047) is 97.20%, determined by SEC-HPLC.

Gen1047 bound to B7-H4 protein, and then rebounded to secondary antibodies (Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Gen1047 bound human B7-H4 Protein-His, and the EC50 was 0.07805 nM.

Gen1047 bound to huB7-H4 CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1047 bound to huB7-H4 CHO-K cells, and the EC50 was 6.894 nM.

Gen1047 bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1047 bound to huCD3e-jurkat cells, and the EC50 was 132.600 nM.

Co-incubation of Gen1047 with Jurkat cells, then with the addition of huB7H4 CHO-k cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Gen1047 was able to activate the NF-AT signaling pathway.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.