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Anti-BCMA/CD3 Reference Antibody (Emb-06)
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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.
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The detected molecular weight of Anti-CD3 & BCMA Reference Antibody (Emb-06) is 241.13 kDa.

Anti-BCMA & CD3 Reference Antibody (Emb-06) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-BCMA & CD3 Reference Antibody (Emb-06) is 91.32%, determined by SEC-HPLC.

Emb-06 bound to BCMA protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Emb-06 bound to huBCMA-ECD-His, and the EC50 was 0.015 nM.

Emb-06 bound to huBCMA-HEK293 cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Emb-06 bound to huBCMA-HEK293 cells, and the EC50 was 1.098 nM.

Emb-06 bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Emb-06 bound to huCD3e-jurkat cells, and the EC50 was 69.310 nM.

Co-incubation of Emb-06 with Jurkat cells, then with the addition of huBCMA-HEK293 cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.As shown in fig, Emb-06 was able to activate the NF-AT signaling pathway, and the EC50 was 0.181 nM.

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-80°C for 2 years under sterile conditions;-20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
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