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Anti-CD28/EGFR Reference Antibody (Regn7075)
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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.
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The detected molecular weight of Anti-EGFR & CD28 Reference Antibody (Regn7075) is 144.98 kDa.

Anti-CD28 & EGFR Reference Antibody (Regn7075) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-CD28 & EGFR Reference Antibody (Regn7075) is 99.31%, determined by SEC-HPLC.

Regn7075 bound to CD28 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Regn7075 bound to huCD28-His, and the EC50 was 0.631 nM.

Regn7075 bound to EGFR protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Regn7075 bound to huEGFR-His, and the EC50 was 0.031 nM.

Regn7075 bound to EGFR-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Regn7075 bound to EGFR-CHO-K cells, and the EC50 was 2.363 nM.

Regn7075 bound to hu CD28-FL-CHO cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Regn7075 bound to hu CD28-FL-CHO cells, and the EC50 was 13.190 nM.

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-80°C for 2 years under sterile conditions;-20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
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