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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

Anti-4-1BB & CD40 Reference Antibody (Gen1042) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-4-1BB & CD40 Reference Antibody (Gen1042) is 96.80%, determined by SEC-HPLC.

Gen1042 bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Gen1042 bound to hu-4-1BB-His, and the EC50 was 0.015 nM.

Gen1042 bound to CD40 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Gen1042 bound to hu-CD40-His, and the EC50 was 0.066 nM.

Gen1042 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1042 bound to hu4-1BB-CHO-K cells, and the EC50 was 4.119 nM.

Gen1042 bound to huCD40-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1042 bound to huCD40-CHO-K cells, and the EC50 was 4.535 nM.

Co-incubation of Gen1042 with 4-1BB-NF-κB-Jurkat cells, then with the addition of huCD40-CHO-K cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Gen1042 was able to activate the NF-κB signaling pathway, and the EC50 was 0.234 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.