RO7122290 是一种双特异性融合蛋白，携带分裂三聚体 4-1BB (CD137) 配体和成纤维细胞活化蛋白 α (FAP) 结合位点。O7122290 是一种 FAP-α 靶向 4-1BB 激动剂，可共刺激 T 细胞，从而提高 FAP 表达肿瘤中肿瘤细胞的杀伤力。

For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

本产品仅用作科学研究！

Anti-4-1BB & HER2/neu Reference Antibody (Yh32367) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-4-1BB & HER2/neu Reference Antibody (Yh32367) is 98.99%, determined by SEC-HPLC.

Yh32367 bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-4-1BB-His, and the EC50 was 0.007 nM.

Yh32367 bound to Her2 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-Her2-His, and the EC50 was 0.009 nM.

Yh32367 bound to BT474 cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to BT474 cells, and the EC50 was 1.889 nM.

Yh32367 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to hu4-1BB-CHO-K cells, and the EC50 was 5.701 nM.

Co-incubation of Yh32367 with BT474 cell and PBMCs for 4 hours, then LDH was detected to evaluate the ADCC activity of Yh32367. As shown in fig, Yh32367 has ADCC activity, and the IC50 was 0.054 nM.

-80°C for 2 years under sterile conditions；-20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles.