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Anti-BCMA/BCMA/CD3 Reference Antibody (TNB-383B)
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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.
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The detected molecular weight of Anti-BCMA & BCMA & CD3 Reference Antibody (TNB-383B) is 123.67 kDa

Anti-BCMA & BCMA & CD3 Reference Antibody (TNB-383B) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-BCMA & BCMA & CD3 Reference Antibody (TNB-383B) is 97.60%, determined by SEC-HPLC.

TNB-383B bound to BCMA protein, and then rebounded to secondary antibodies (Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Tnb-383B bound to huBCMA-ECD-His, and the EC50 was 0.063 nM.

TNB-383B bound to huBCMA-HEK293 cells, and then rebounded to fluorescent secondary antibodies (Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, TNB-383B bound to huBCMA-HEK293 cells, and the EC50 was 0.588 nM.

Co-incubation of TNB-383B with Jurkat cells, then with the addition of huBCMA-HEK293 cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.As shown in fig, TNB-383B was able to activate the NF-AT signaling pathway, and the EC50 was 0.152 nM.

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-80°C for 2 years under sterile conditions;-20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
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