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Anti-PD-L1/VEGF Reference Antibody (Sotiburafusp alfa)
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For Powder, reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.
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Anti-PD-L1 & VEGF Reference Antibody (Hb0025)on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

The purity of Anti-PD-L1 & VEGF Reference Antibody (Hb0025)is 100%, determined by SEC-HPLC.

Hb0025 bound to VEGF protein, and then rebounded to secondary antibodies(Anti Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Hb0025 bound to huVEGFA-His , and the EC50 was 0.022 nM.

Hb0025 bound to PD-L1 protein, and then rebounded to secondary antibodies(Anti-Human-κ+λ-HRP) , and read OD450. As shown in fig, Hb0025 bound to huPD-L1-Fc , and the EC50 was 0.044 nM.

Hb0025 bound to huPD-L1-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Hb0025 bound to huPD-L1-CHO-K cells, and the EC50 was 2.025 nM.

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-80°C for 2 years under sterile conditions;-20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
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