GM130兔单克隆抗体 GM130 Rabbit pAb兔单抗(38971ES)
产品应用
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi.
WB: 1/1000-1/20000 IHC: 1/100-1/1000 IF: 1/50-1/200 IP: 1/50
Western
Western blot analysis of GM130 in HeLa, lysates using GM130 antibody.

Western blot analysis of GM130 in (1) HeLa lysates; (2) MCF-7 lysates using GM130 antibody.
Immunohistochemistry
Immunohistochemistry analysis of paraffin-embedded Human cervix carcinoma using GM130 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence micrograph of mouse liver from paraffin-embedded sections using GM130 Rabbit mAb , dilution 1/100 and Goat Anti-Rabbit IgG H+L (594,dilution 1/300) (Red). Nuclear were stained with DAPI (Blue). Heat-induced epitope retrieval was performed using Tris-EDTA buffer (pH 9.0) prior to staining. Objective: 20X .
-25 ~ -15℃保存,收到货之后有效期1年,避免反复冻融。
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