Smad4 transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/100-1/200  IF: 1/50-1/200  FC: 1/50-1/100

Flow

Flow cytometric analysis of K562 cells stained with SMAD4 antibody (green) and negative control (purple).

Immunohistochemical

Immunohistochemical analysis of paraffin-embedded Human tonsils using Smad4 antibody, with DAB staining.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。