Suppression of PtrDUF579-3 Expression Causes Structural Changes of the Glucuronoxylan in Populus
Song Dongliang, Gui Jinshan, Liu Chenchen, Sun Jiayan, Li Laigeng
Journal：Frontiers in Plant Science
IF：4.5
DOI：10.3389/fpls.2016.00493
PMID：
Published：2016-04-11
research field：植物生物学分子遗传学进化生物学生物化学
Abstract
DUF579 (domain unknown function 579) genes have been reported to play diverse roles in cell wall biosynthesis, such as in glucuronoxylan (GX) synthesis. As GX is a major type of hemicelluloses in hard wood species, how DUF579 genes function in wood formation remains to be demonstrated in planta. This study reports a Populus DUF579 gene, PtrDUF579-3, which is characterized for its function in wood cell wall formation. PtrDUF579-3 is localized in Golgi apparatus and catalyzes methylation of the glucuronic acid (GlcA) in GX biosynthesis. Suppression of PtrDUF579-3 expression in Populus caused a reduction in both the GlcA side chain number and GlcA side chain methylation on the GX backbone. The modified GX polymer through PtrDUF579-3 suppression was more susceptible to acid treatment and the PtrDUF579-3 suppressed plants displayed enhanced cellulose digestibility. These results suggest that PtrDUF579-3 is involved in GX biosynthesis and GX structure can be modified through PtrDUF579-3 suppression in Populus.
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