略阳乌鸡肝肽通过调节肠道菌群和紧密连接蛋白缓解DSS诱导溃疡性结肠炎

Abstract

Ulcerative colitis (UC), an inflammatory bowel disease with long-term medication side effects, requires safe therapies. Lueyang black-bone chicken liver peptides (BLP) were prepared by hydrolyzing its liver with 1 % alkaline protease for 1 h. Peptides with a molecular weight of less than 1.5 kDa account for 77.6 %. The hydrophobic and basic amino acids of BLP accounted for 47.20 % and 11.75 %, respectively. In dextran sulfate sodium (DSS)-induced UC mice, BLP increased body weight, reduced DAI scores, restored colon length, ameliorated tissue damage, decreased inflammatory cytokines, and upregulated tight junction proteins (claudin-1, occludin and ZO-1). Meanwhile, BLP increased the abundance of probiotics ( Akkermansia , Dubosiella, Coriobacteriaceae_ UCG-002, and Bifidobacterium ). Among them, Akkermansia promoted the synthesis of key metabolites, such as 5-hydroxyindole-3-acetic acid, picolinic acid, prostaglandin D2, lipoxin A4 and chenodeoxycholic acid, thereby alleviating UC. This study provides a new evidence for UC relief and a basis for high-value utilization of chicken liver.