Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

推荐稀释比 WB: 1/1000-1/2000  IHC: 1/100-1/200  IF: 1/50-1/200  IP: 1/30

Western

Western blot analysis of HistoneH2A.X Phosphorylation in Jurkat lysates treated with etoposide using Phospho-Histone H2A.X (Ser139) antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded mouse liver using Phospho-Histone H2A.X (S139) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunofluorescence

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in HeLa treated with H2O2 using Phospho-Histone H2A.X (S139) antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。