Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/50-1/100  IF: 1/50-1/200  ELISA: 1/10000

Western

Western blot analysis of HDAC5 in various lysates using HDAC5 antibody.

Western

Western blot analysis of HDAC5 in K562 lysates using HDAC5 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using HDAC5 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western

Western blot analysis of HDAC5 in colo lysates using HDAC5 antibody.The lane on the right is blocked with the synthesized peptide.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。