分子生物学
IVD分子诊断
细胞培养与分析
蛋白研究
细胞因子
重组蛋白
抗体
高通量测序建库
病原检测UCF系列
生物医药
工具酶
抑制剂激活剂与常用试剂
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耗材
SUMO Conjugating Enzyme UBC9 Rabbit mAb
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The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain.
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推荐稀释比 WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
应用案例

Immunocytochemistry

Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using SUMO Conjugating Enzyme UBC9 antibody,and DAPI(blue).


Western

Western blot analysis of UBE2I/UBC9 in 3T3, PC-12, MEF lysates using UBE2I/UBC9 antibody.


Western

Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody


Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.


存储条件

-25 ~ -15℃保存,收到货之后有效期1年,避免反复冻融。

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