eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Western

Western blot analysis of EIF4E in Hela (1), HEK293 (2) and K562 (3) cell lysate using EIF4E antibody.

Immunocytochemistry

Immunocytochemistry analysis of EIF4E in GC-7901 cells using EIF4E antibody (green),and DAPI(blue). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

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