May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity).

Western

Western blot analysis of Klotho in Human fetal kidney lysates using Klotho antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human fetal kidney using klotho antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.