β-actin（β-肌动蛋白）是已知的肌动蛋白六种异构体中的一种，是由 375 个氨基酸组成的细胞骨架蛋白，分子量大小为 42 kDa 左右。广泛分布于细胞浆中，表达量非常丰富。β-actin 作为一种管家基因编码表达的蛋白（Housekeeping Protein），在不同物种之间高度保守。因此，在大多数组织和细胞中常被用作内参，即内部参照（Intern Control）。普遍应用于 Western Blot 实验来校正蛋白质定量、上样过程中存在的误差，确保实验的准确性。
稀释比 WB:1/5000-1/10000; IHC:1/50-1/200; IF:1/100-1/200;
 | Western blot analysis of beta Actin in (1)HeLa lysates; (2) Human fetal kidney lysates; (3) NIH/3T3 lysates; (4) PC12 lysates using beta Actin antibody. |
 | Immunofluorescence analysis of beta Actin in Hela using beta Actin antibody. |
 | Immunohistochemistry analysis of paraffinembedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart using beta Actin antibody. |
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