His标签是由6个组氨酸(His-His-His-His-His-His)组成的短肽,可与多种蛋白形成融合蛋白,基于组氨酸的咪唑环与金属离子(如镍离子)起化学作用而生成螯合的原理,可以利用His-Tag标签纯化His融合蛋白。由于分子量较小,并且较容易分离和纯化,His融合标签与其他标签相比有很多明显优势,是目前融合标签中使用最为广泛的一种。本品为HRP标记的His-tag抗体,可特异性识别来源于多种物种(如细菌、昆虫、哺乳动物)的位于N端或C端His的融合蛋白。抗体具有高灵敏度,高特异性和高亲和力。
建议稀释浓度:WB:1:1000-1:10000;
-25 ~ -15℃分装保存,尽量避免反复冻融。
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