His标签是由6个组氨酸(His-His-His-His- His-His)组成的短肽，可与多种蛋白形成融合蛋白，基于组氨酸的咪唑环与金属离子（如镍离子）起化学作用而生成螯合的原理，可以利用His-Tag标签纯化His融合蛋白。由于分子量较小，并且较容易分离和纯化，His融合标签与其他标签相比有很多明显优势，是目前融合标签中使用最为广泛的一种。翌圣生产的抗His-tag抗体可特异性的识别来源于多种物种（如细菌、昆虫、哺乳动物）的位于N端、C端His的融合蛋白，可用于融合蛋白表达的检测及其后续纯化。抗体具有高灵敏度，高特异性和高亲和力。
稀释比 WB: 1/1000-1/10000；ELISA: 1/5000-1/20000
| Western blot | |
 | Western Blot analysis of His Tag in HIS-protein lysates using His-tag antibody. |
冰袋运输，-25~-15 ℃保存。
[1] Wang Y, Hou Y, Qiu J, et al. Abscisic acid promotes jasmonic acid biosynthesis via a 'SAPK10-bZIP72-AOC' pathway to synergistically inhibit seed germination in rice (Oryza sativa). New Phytol. 2020;228(4):1336-1353. doi:10.1111/nph.16774(IF:8.512)
[2] Zou G, Bao D, Wang Y, et al. Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase. Bioresour Technol. 2021;319:124119. doi:10.1016/j.biortech.2020.124119(IF:7.539)
[3] Chen X, Yang L, Huang R, Li S, Jia Q. Matrix metalloproteinases are involved in eclosion and wing expansion in the American cockroach, Periplaneta americana. Insect Biochem Mol Biol. 2021;131:103551. doi:10.1016/j.ibmb.2021.103551(IF:4.714)
[4] Lin T, Xing J, Tang X, Sheng X, Chi H, Zhan W. Development and Evaluation of a Bicistronic DNA Vaccine against Nervous Necrosis Virus in Pearl Gentian Grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus). Vaccines (Basel). 2022;10(6):946. Published 2022 Jun 14. doi:10.3390/vaccines10060946(IF:4.422)
[5] Cao H, Hua D, Zhang H, et al. Oral immunization of recombinant Saccharomyces cerevisiae expressing fiber-2 of fowl adenovirus serotype 4 induces protective immunity against homologous infection. Vet Microbiol. 2022;271:109490. doi:10.1016/j.vetmic.2022.109490(IF:3.293)
[6] Zhai H, Shi J, Sun R, et al. The superposition anti-viral activity of porcine tri-subtype interferon expressed by Saccharomyces cerevisiae. Vet Microbiol. 2021;259:109150. doi:10.1016/j.vetmic.2021.109150(IF:3.293)
[7] Cheng D, Lv H, Yao Y, et al. Roles of the Site 2 Protease Eep in Staphylococcus aureus. J Bacteriol. 2020;202(15):e00046-20. Published 2020 Jul 9. doi:10.1128/JB.00046-20(IF:3.006)
