融合蛋白表达中通常在蛋白N-端或C-端加上特定蛋白或多肽序列作为一种抗原表位（标签蛋白）以便于目的蛋白后续的定位、表达、检测及纯化。Glutathione S-Transferase （即谷胱甘肽S-转移酶，GST）是应用非常广泛的一种蛋白标签，来源于Schistosoma japonicum，分子量为26kDa，可应用于各种融合蛋白，可在大肠杆菌和酵母菌等宿主细胞中表达，用GST亲和柱可简便地分离融合蛋白。 GST标签有助于保护重组蛋白免受胞外蛋白酶的降解并提高其稳定性。GST抗体可以用于GST融合蛋白后续的表达、细胞内定位，纯化、以及定性或定量分析。
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[1] Kang Y, Chen L. Structure and mechanism of NALCN-FAM155A-UNC79-UNC80 channel complex. Nat Commun. 2022;13(1):2639. Published 2022 May 12. doi:10.1038/s41467-022-30403-7(IF:14.919)
[2] Kang Y, Wu JX, Chen L. Structure of voltage-modulated sodium-selective NALCN-FAM155A channel complex. Nat Commun. 2020;11(1):6199. Published 2020 Dec 3. doi:10.1038/s41467-020-20002-9(IF:12.121)
[3] Tang C, Wu X, Ren Q, Yao M, Xu S, Yan Z. Hedgehog signaling is controlled by Rac1 activity. Theranostics. 2022;12(3):1303-1320. Published 2022 Jan 1. doi:10.7150/thno.67702(IF:11.556)
[4] Han L, Zhang F, Liu Y, et al. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection. Clin Transl Med. 2022;12(6):e850. doi:10.1002/ctm2.850(IF:11.492)
[5] Ai H, Cao Y, Jain A, et al. The ferroxidase LPR5 functions in the maintenance of phosphate homeostasis and is required for normal growth and development of rice. J Exp Bot. 2020;71(16):4828-4842. doi:10.1093/jxb/eraa211(IF:5.908)
[6] Wu Q, Huang Y, Gu L, Chang Z, Li GM. OTUB1 stabilizes mismatch repair protein MSH2 by blocking ubiquitination. J Biol Chem. 2021;296:100466. doi:10.1016/j.jbc.2021.100466(IF:5.157)
[7] Wang D, Tan KS, Arias-Moreno X, Tan W, Cheng G. Sirt3 increases CNPase enzymatic activity through deacetylation and facilitating substrate accessibility. Biochem Biophys Res Commun. 2021;571:181-187. doi:10.1016/j.bbrc.2021.07.079(IF:3.575)
