Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Flow cytometric analysis of Glutamic-oxaloacetic transaminase 2 expression in HepG2 cells using Glutamic-oxaloacetic transaminase 2 antibody . Green, isotype control; red, Glutamic-oxaloacetic transaminase 2.

Immunocytochemical staining of HepG2 cells with Glutamic-oxaloacetic transaminase 2 antibody . Nuclei were stained blue with DAPI; Glutamic-oxaloacetic transaminase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Glutamic-oxaloacetic transaminase 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glutamic-oxaloacetic transaminase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Glutamic-oxaloacetic transaminase 2 antibody . Glutamic-oxaloacetic transaminase 2 expression in wild type (WT) and Glutamic-oxaloacetic transaminase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Glutamic-oxaloacetic transaminase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Store at -20℃ for one year.